New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing

doi:10.1016/j.jhazmat.2023.131271

	Dai, Yeliang 1,2,3; Li, Jibing 1,2,3; Yang, Xiumin 1; Wang, Shuang 1,2,3; Zhao, Xuan 1,2,3; Wang, Yujie 4; Zhang, Dayi 5; Luo, Chunling1,2,3 ; Zhang, Gan1,2,3
	New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing
刊名	JOURNAL OF HAZARDOUS MATERIALS
ISSN号	0304-3894
	2023
卷号	452 页码:131271
DOI	10.1016/j.jhazmat.2023.131271
语种	英语
摘要	Autochthonous fungal bioaugmentation (AFB) is considered a reliable bioremediation approach for polycyclic aromatic hydrocarbon (PAH) contamination, but little is known about its mechanisms in contaminated soils. Here, a microcosm experiment was performed to explore the AFB mechanisms associated with two highly efficient phenanthrene degrading agents of fungi (with laccase-producing Scedosporium aurantiacum GIG-3 and non-laccase-producing Aspergillus fumigatus LJD-29), using stable-isotope-probing (SIP) and high-throughput sequencing. The results showed that each fungus markedly improved phenanthrene removal, and microcosms with both fungi exhibited the best phenanthrene removal performance among all microcosms. Additionally, AFB markedly shifted the composition of the microbial community, particularly the phenanthrene-degrading bacterial taxa. Interestingly, based on SIP results, strains GIG-3 and LJD-29 did not assimilate phenanthrene directly during AFB, but instead played key roles in the preliminary decomposition of phenanthrene though secretion of different extracellular enzymes to oxidize the benzene ring (GIG-3 bioaugmentation with laccase, and LJD-29 bioaugmentation with manganese and lignin peroxidases). In addition, all functional degraders directly involved in phenanthrene assimilation were indigenous bacteria, while native fungi rarely participated in the direct phenanthrene mineralization. Our findings provide a new mechanism of AFB with multiple fungi, and support AFB as a promising strategy for the in situ bioremediation of PAH-contaminated soil. ? 2023 Elsevier B.V.
收录类别	SCI
引用统计	正在获取...
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/80313
专题	有机地球化学国家重点实验室
作者单位	1.State Key Laboratory of Organic Geochemistry and Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou; 510640, China 2.CAS Center for Excellence in Deep Earth Science, Guangzhou; 510640, China 3.University of Chinese Academy of Sciences, Beijing; 100039, China 4.School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou; 510006, China 5.College of New Energy and Environment, Jilin University, Changchun; 130021, China
推荐引用方式 GB/T 7714	Dai, Yeliang,Li, Jibing,Yang, Xiumin,et al. New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing[J]. JOURNAL OF HAZARDOUS MATERIALS,2023,452:131271.
APA	Dai, Yeliang.,Li, Jibing.,Yang, Xiumin.,Wang, Shuang.,Zhao, Xuan.,...&Zhang, Gan.(2023).New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing.JOURNAL OF HAZARDOUS MATERIALS,452,131271.
MLA	Dai, Yeliang,et al."New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing".JOURNAL OF HAZARDOUS MATERIALS 452(2023):131271.

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