Highly Sensitive Aptasensor for Trace Arsenic(III) Detection Using DNAzyme as the Biocatalytic Amplifier

doi:10.1021/acs.analchem.8b05466

GIG OpenIR

	Zeng, Lingwen 1; Zhou, Danhua 2; Gong, Junyu 1; Liu, Chengshuai 3; Chen, Junhua 2
	Highly Sensitive Aptasensor for Trace Arsenic(III) Detection Using DNAzyme as the Biocatalytic Amplifier
刊名	ANALYTICAL CHEMISTRY
ISSN号	0003-2700
	2019-02-05
卷号	91 期号:3 页码:1724-1727
DOI	10.1021/acs.analchem.8b05466
语种	英语
WOS研究方向	Chemistry
摘要	A highly sensitive fluorescence biosensing system was designed for the detection of trace amounts of arsenic(III) (As3+) based on target-triggered successive signal amplification strategy. The specific recognition between the target As' and the aptamer sequence results in the release of the blocking DNA to trigger the subsequent signal amplification steps. Exonuclease III (Exo III) -mediated DNA recycling digest process is introduced into the sensing system to generate numerous Mg2+-dependent DNAzymes. After magnetic separation, the active DNAzyme with multiple turnovers could catalyze the continuous cleavage of the fluorophore-quencher-functionalized substrate strands, thus yielding a significantly amplified fluorescence signal for target detection. Due to the synergetic signal amplification of Exo III and DNAzyme, the fluorescent biosensor exhibits ultrasensitivity for As3+ monitoring, with a detection limit of 2 pM. Our established biosensor also displays excellent selectivity toward the target As3+ and has been successfully applied to the determination of As3+ in water samples with satisfactory accuracy. This sensing platform can be developed as a universal approach for the fast, sensitive, and accurate detection of aptamer-binding molecules.
WOS记录号	WOS:000458220300011
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/52760
通讯作者	Chen, Junhua
作者单位	1.Foshan Univ, Sch Food Sci & Engn, Foshan 528000, Peoples R China 2.Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China 3.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Lingwen,Zhou, Danhua,Gong, Junyu,et al. Highly Sensitive Aptasensor for Trace Arsenic(III) Detection Using DNAzyme as the Biocatalytic Amplifier[J]. ANALYTICAL CHEMISTRY,2019,91(3):1724-1727.
APA	Zeng, Lingwen,Zhou, Danhua,Gong, Junyu,Liu, Chengshuai,&Chen, Junhua.(2019).Highly Sensitive Aptasensor for Trace Arsenic(III) Detection Using DNAzyme as the Biocatalytic Amplifier.ANALYTICAL CHEMISTRY,91(3),1724-1727.
MLA	Zeng, Lingwen,et al."Highly Sensitive Aptasensor for Trace Arsenic(III) Detection Using DNAzyme as the Biocatalytic Amplifier".ANALYTICAL CHEMISTRY 91.3(2019):1724-1727.