Knowledge Management System Of Guangzhou Institute of Geochemistry,CAS
Ping, Wangfang1,2,3; Hu, Jian4; Hu, Gongcheng1,3; Song, Yawei1,3; Xia, Qing1,3; Yao, Mingze1,3; Gong, Shixin1,3; Jiang, Cizhong4; Yao, Hongjie1,3 | |
Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs | |
刊名 | CELL DEATH & DISEASE
![]() |
ISSN号 | 2041-4889 |
2018-02-07 | |
卷号 | 9页码:13 |
DOI | 10.1038/s41419-017-0234-x |
语种 | 英语 |
WOS研究方向 | Cell Biology |
摘要 | Induced pluripotent stem cells can be derived from somatic cells through ectopic expression of transcription factors or chemical cocktails. Chemical iPSCs (C-iPSCs) and OSKM-iPSCs (4F-iPSCs) have been suggested to have similar characteristics to mouse embryonic stem cells (mESCs). However, their epigenetic equivalence remains incompletely understood throughout the genome. In this study, we have generated mouse C-iPSCs and 4F-iPSCs, and further compared the genome-wide DNA methylomes of C-iPSCs, 4F-iPSCs, and mESCs that were maintained in 2i and LIF. Three pluripotent stem cells tend to be low methylated overall, however, DNA methylations in some specific regions (such as retrotransposons) are cell type-specific. Importantly, C-iPSCs are more hypomethylated than 4F-iPSCs. Bisulfite sequencing indicated that DNA methylation status in several known imprinted clusters, such as: Dlk1-Dio3 and Peg12-Ube3a, in C-iPSCs are closer to those of mESCs than 4F-iPSCs. Overall, our data demonstrate the reprogramming methods-dependent epigenetic differences of C-iPSCs and 4F-iPSCs and reveal that C-iPSCs are more hypomethylated than OSKM-integrated iPSCs. |
WOS记录号 | WOS:000427401400029 |
收录类别 | SCI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.gig.ac.cn/handle/344008/44473 |
通讯作者 | Jiang, Cizhong; Yao, Hongjie |
作者单位 | 1.Guangzhou Med Univ, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Guangzhou Inst Bi, CAS Key Lab Regenerat Biol,Joint Sch Life Sci, Guangzhou, Guangdong, Peoples R China 2.Anhui Univ, Inst Hlth Sci, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou, Guangdong, Peoples R China 4.Tongji Univ, Collaborat Innovat Ctr Brain Sci, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Ping, Wangfang,Hu, Jian,Hu, Gongcheng,et al. Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs[J]. CELL DEATH & DISEASE,2018,9:13. |
APA | Ping, Wangfang.,Hu, Jian.,Hu, Gongcheng.,Song, Yawei.,Xia, Qing.,...&Yao, Hongjie.(2018).Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs.CELL DEATH & DISEASE,9,13. |
MLA | Ping, Wangfang,et al."Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs".CELL DEATH & DISEASE 9(2018):13. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论