Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs

doi:10.1038/s41419-017-0234-x

GIG OpenIR

	Ping, Wangfang 1,2,3; Hu, Jian 4; Hu, Gongcheng 1,3; Song, Yawei 1,3; Xia, Qing 1,3; Yao, Mingze 1,3; Gong, Shixin 1,3; Jiang, Cizhong 4; Yao, Hongjie 1,3
	Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs
刊名	CELL DEATH & DISEASE
ISSN号	2041-4889
	2018-02-07
卷号	9 页码:13
DOI	10.1038/s41419-017-0234-x
语种	英语
WOS研究方向	Cell Biology
摘要	Induced pluripotent stem cells can be derived from somatic cells through ectopic expression of transcription factors or chemical cocktails. Chemical iPSCs (C-iPSCs) and OSKM-iPSCs (4F-iPSCs) have been suggested to have similar characteristics to mouse embryonic stem cells (mESCs). However, their epigenetic equivalence remains incompletely understood throughout the genome. In this study, we have generated mouse C-iPSCs and 4F-iPSCs, and further compared the genome-wide DNA methylomes of C-iPSCs, 4F-iPSCs, and mESCs that were maintained in 2i and LIF. Three pluripotent stem cells tend to be low methylated overall, however, DNA methylations in some specific regions (such as retrotransposons) are cell type-specific. Importantly, C-iPSCs are more hypomethylated than 4F-iPSCs. Bisulfite sequencing indicated that DNA methylation status in several known imprinted clusters, such as: Dlk1-Dio3 and Peg12-Ube3a, in C-iPSCs are closer to those of mESCs than 4F-iPSCs. Overall, our data demonstrate the reprogramming methods-dependent epigenetic differences of C-iPSCs and 4F-iPSCs and reveal that C-iPSCs are more hypomethylated than OSKM-integrated iPSCs.
WOS记录号	WOS:000427401400029
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/44473
通讯作者	Jiang, Cizhong; Yao, Hongjie
作者单位	1.Guangzhou Med Univ, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Guangzhou Inst Bi, CAS Key Lab Regenerat Biol,Joint Sch Life Sci, Guangzhou, Guangdong, Peoples R China 2.Anhui Univ, Inst Hlth Sci, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou, Guangdong, Peoples R China 4.Tongji Univ, Collaborat Innovat Ctr Brain Sci, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Ping, Wangfang,Hu, Jian,Hu, Gongcheng,et al. Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs[J]. CELL DEATH & DISEASE,2018,9:13.
APA	Ping, Wangfang.,Hu, Jian.,Hu, Gongcheng.,Song, Yawei.,Xia, Qing.,...&Yao, Hongjie.(2018).Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs.CELL DEATH & DISEASE,9,13.
MLA	Ping, Wangfang,et al."Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs".CELL DEATH & DISEASE 9(2018):13.