Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass Thalassia hemprichii in Coral Reef Ecosystems

doi:10.3389/fmicb.2018.00007

GIG OpenIR

	Ling, Juan 1; Lin, Xiancheng 1,2; Zhang, Yanying 1,3; Zhou, Weiguo 1,2; Yang, Qingsong 1,2; Lin, Liyun 1,2; Zeng, Siquan 1,2; Zhang, Ying 1,2; Wang, Cong 1,2; Ahmad, Manzoor 1,2; Long, Lijuan 1; Dong, Junde 1,3
	Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass Thalassia hemprichii in Coral Reef Ecosystems
刊名	FRONTIERS IN MICROBIOLOGY
ISSN号	1664-302X
	2018-01-25
卷号	9 页码:13
DOI	10.3389/fmicb.2018.00007
语种	英语
WOS研究方向	Microbiology
摘要	Seagrasses in coral reef ecosystems play important ecological roles by enhancing coral reef resilience under ocean acidification. However, seagrass primary productivity is typically constrained by limited nitrogen availability. Ammonia oxidation is an important process conducted by ammonia-oxidizing archaea (AOA) and bacteria (AOB), yet little information is available concerning the community structure and potential activity of seagrass AOA and AOB. Therefore, this study investigated the variations in the abundance, diversity and transcriptional activity of AOA and AOB at the DNA and transcript level from four sample types: the leaf, root, rhizosphere sediment and bulk sediment of seagrass Thalassia hemprichii in three coral reef ecosystems. DNA and complementary DNA (cDNA) were used to prepare clone libraries and DNA and cDNA quantitative PCR (qPCR) assays, targeting the ammonia monooxygenase-subunit (amoA) genes as biomarkers. Our results indicated that the closest relatives of the obtained archaeal and bacterial amoA gene sequences recovered from DNA and cDNA libraries mainly originated from the marine environment. Moreover, all the obtained AOB sequences belong to the Nitrosomonadales cluster. Nearly all the AOA communities exhibited higher diversity than the AOB communities at the DNA level, but the qPCR data demonstrated that the abundances of AOB communities were higher than that of AOA communities based on both DNA and RNA transcripts. Collectively, most of the samples shared greater community composition similarity with samples from the same location rather than sample type. Furthermore, the abundance of archaeal amoA gene in rhizosphere sediments showed significant relationships with the ammonium concentration of sediments and the nitrogen content of plant tissue (leaf and root) at the DNA level (P < 0.05). Conversely, no such relationships were found for the AOB communities. This work provides new insight into the nitrogen cycle, particularly nitrification of seagrass meadows in coral reef ecosystems.
关键词	seagrass ammonia-oxidizing archaea and bacteria ammonia monooxygenase subunit A (amoA) cDNA community structure coral reef ecosystems
WOS记录号	WOS:000423362400001
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/43925
通讯作者	Dong, Junde
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Trop Marine Biol Res Stn Hainan, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Ling, Juan,Lin, Xiancheng,Zhang, Yanying,et al. Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass Thalassia hemprichii in Coral Reef Ecosystems[J]. FRONTIERS IN MICROBIOLOGY,2018,9:13.
APA	Ling, Juan.,Lin, Xiancheng.,Zhang, Yanying.,Zhou, Weiguo.,Yang, Qingsong.,...&Dong, Junde.(2018).Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass Thalassia hemprichii in Coral Reef Ecosystems.FRONTIERS IN MICROBIOLOGY,9,13.
MLA	Ling, Juan,et al."Community Composition and Transcriptional Activity of Ammonia-Oxidizing Prokaryotes of Seagrass Thalassia hemprichii in Coral Reef Ecosystems".FRONTIERS IN MICROBIOLOGY 9(2018):13.