A wash-free and label-free colorimetric biosensor for naked-eye detection of aflatoxin B1 using G-quadruplex as the signal reporter

doi:10.1016/j.foodchem.2019.125034

GIG OpenIR

	Wu, Jinghua 1; Zeng, Lingwen 1,2; Li, Nianlong 1; Liu, Chengshuai 3; Chen, Junhua 4
	A wash-free and label-free colorimetric biosensor for naked-eye detection of aflatoxin B1 using G-quadruplex as the signal reporter
刊名	FOOD CHEMISTRY
ISSN号	0308-8146
	2019-11-15
卷号	298 页码:6
DOI	10.1016/j.foodchem.2019.125034
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
摘要	A wash-free and label-free colorimetric biosensor for the amplified detection of aflatoxin B1 (AFB1) has been constructed by the integration of an ingenious hairpin DNA probe with exonuclease III (Exo III)-assisted signal amplification. The presence of the AFB1 activates the continuous cleavage reactions by Exo III toward a hairpin probe, resulting in the autonomous accumulation of numerous free G-quadruplex sequences, which can catalyze the oxidation of 3,3', 5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a colorimetric response. The nakedeye biosensor is ultrasensitive, enabling the visual detection of trace amounts of AFB1 as low as 1 pM without instrumentation. The sensor is robust and can work even when challenged with complex sample matrices such as peanut samples. With the advantages of simple operation, wash-free and label-free format, visible and intuitive output, and low cost, the naked-eye based colorimetric biosensor is expected to have potential applications for in-field detection of AFB1.
关键词	Aflatoxin B1 Visible biosensor G-quadruplex Signal amplification Peanut samples
WOS记录号	WOS:000477667200009
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/41357
通讯作者	Chen, Junhua
作者单位	1.Foshan Univ, Sch Food Sci & Engn, Foshan 528000, Peoples R China 2.Wuhan Acad Agr Sci, Inst Environm & Food Safety, Wuhan 430065, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Guizhou, Peoples R China 4.Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jinghua,Zeng, Lingwen,Li, Nianlong,et al. A wash-free and label-free colorimetric biosensor for naked-eye detection of aflatoxin B1 using G-quadruplex as the signal reporter[J]. FOOD CHEMISTRY,2019,298:6.
APA	Wu, Jinghua,Zeng, Lingwen,Li, Nianlong,Liu, Chengshuai,&Chen, Junhua.(2019).A wash-free and label-free colorimetric biosensor for naked-eye detection of aflatoxin B1 using G-quadruplex as the signal reporter.FOOD CHEMISTRY,298,6.
MLA	Wu, Jinghua,et al."A wash-free and label-free colorimetric biosensor for naked-eye detection of aflatoxin B1 using G-quadruplex as the signal reporter".FOOD CHEMISTRY 298(2019):6.