A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates

doi:10.1371/journal.pone.0194010

GIG OpenIR

	Zhao, Jin 1,2,7; Xia, Haixiong 1; Yu, Tingyu 3; Jin, Lu 1; Li, Xuehua 1; Zhang, Yinghui 4; Shu, Liping 1; Zeng, Lingwen 4,5,6; He, Zhixu 1,2
	A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates
刊名	PLOS ONE
ISSN号	1932-6203
	2018-04-20
卷号	13 期号:4 页码:11
DOI	10.1371/journal.pone.0194010
语种	英语
WOS研究方向	Science & Technology - Other Topics
摘要	Vanillin (4-hydroxy-3-methoxybenzaldehyde), a food additive with rich milk flavor, is commonly used in the food, beverage and cosmetic industries. However, excessive consumption of vanillin may cause liver and kidney damage. Therefore, methods for detecting and controlling the level of vanillin in food, especially in infant powder, have important practical significance. In this study, we established a colorimetric assay for vanillin detection. The detection was performed under high-temperature and acidic conditions, which can induce the reaction of the aldehyde group of vanillin with the amino group of o-toluidine. The resulting product had a maximum absorption at 363 nm, which was quantified by a UV spectrophotometer. This assay had a limit of detection (LOD) of 1 pg mL(-1) and a linear range between 1 mu g mL(-1) and 100 mu g mL(-1). The average recoveries at three spiked levels were in the range from 91.1% to 101.6% with a relative standard deviation (RSD) of 4.62% similar to 7.27%.
WOS记录号	WOS:000430638900004
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.gig.ac.cn/handle/344008/38116
通讯作者	Zeng, Lingwen; He, Zhixu
作者单位	1.Guizhou Med Univ, Sch Basic Med, Dept Immunol, Tissue Engn & Stem Cell Res Ctr,Guizhou Prov Key, Guiyang, Guizhou, Peoples R China 2.Guizhou Med Univ, State Key Lab Med Plant Efficacy & Utilizat, Affiliated Hosp, Guiyang, Guizhou, Peoples R China 3.Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou, Guangdong, Peoples R China 4.Foshan Univ, Sch Food Sci & Engn, Foshan, Guangdong, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Biomed, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou, Guangdong, Peoples R China 6.Chinese Acad Sci, Guangzhou Inst Hlth, Guangzhou, Guangdong, Peoples R China 7.Guizhou Med Univ, Tissue Engn & Stem Cell Res Ctr, Guizhou Prov Key Lab Regenerat Med, Guiyang, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jin,Xia, Haixiong,Yu, Tingyu,et al. A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates[J]. PLOS ONE,2018,13(4):11.
APA	Zhao, Jin.,Xia, Haixiong.,Yu, Tingyu.,Jin, Lu.,Li, Xuehua.,...&He, Zhixu.(2018).A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates.PLOS ONE,13(4),11.
MLA	Zhao, Jin,et al."A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates".PLOS ONE 13.4(2018):11.