Knowledge Management System Of Guangzhou Institute of Geochemistry，CAS
Li, Jibing1,2,4; Luo, Chunling1,2,4 ; Cai, Xixi3; Dai, Yeliang1,2,4; Zhang, Dayi5,6,7; Zhang, Gan1,2
| |
| Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC) | |
| Source Publication | ENVIRONMENT INTERNATIONAL
 |
| ISSN | 0160-4120 |
| 2024-03-01 | |
| Volume | 185Pages:11 |
| DOI | 10.1016/j.envint.2024.108555 |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology |
| Abstract | High-throughput identification and cultivation of functional-yet-uncultivable microorganisms is a fundamental goal in environmental microbiology. It remains as a critical challenge due to the lack of routine and effective approaches. Here, we firstly proposed an approach of stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC) to isolate and characterize the active phenanthrene degraders from petroleumcontaminated soils. From SIP and metagenome, we assembled 13 high-quality metagenomic bins from 13C- DNA, and successfully obtained the genome of an active PHE degrader Achromobacter (genome-MB) from 13C- DNA metagenomes, which was confirmed by gyrB gene comparison and average nucleotide/amino identity (ANI/ AAI), as well as the quantification of PAH dioxygenase and antibiotic resistance genes. Thereinto, we modified the traditional cultivation medium with antibiotics and specific growth factors (e.g., vitamins and metals), and separated an active phenanthrene degrader Achromobacter sp. LJB-25 via directed isolation. Strain LJB-25 could degrade phenanthrene and its identity was confirmed by ANI/AAI values between its genome and genome-MB (>99 %). Our results hinted at the feasibility of SIP-MDC to identify, isolate and cultivate functional-yetuncultivable microorganisms (active phenanthrene degraders) from their natural habitats. Our findings developed a state-of-the-art SIP-MDC approach, expanded our knowledge on phenanthrene biodegradation mechanisms, and proposed a strategy to mine functional-yet-uncultivable microorganisms. |
| Keyword | Stable isotope probing Metagenome binning Cultivation Phenanthrene degrader Achromobacter |
| WOS ID | WOS:001215840600001 |
| Indexed By | SCI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.gig.ac.cn/handle/344008/77051 |
| Collection | 有机地球化学国家重点实验室 |
| Corresponding Author | Zhang, Dayi |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Guangdong Prov Key Lab Environm Protect & Resource, Guangzhou 510640, Peoples R China 3.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.Jilin Univ, Key Lab Groundwater Resources & Environm, Minist Educ, Changchun 130021, Peoples R China 6.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China 7.Shenyang Univ, Key Lab Reg Environm & Ecorestorat, Minist Educ, Shenyang 110044, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Jibing,Luo, Chunling,Cai, Xixi,et al. Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC)[J]. ENVIRONMENT INTERNATIONAL,2024,185:11. |
| APA | Li, Jibing,Luo, Chunling,Cai, Xixi,Dai, Yeliang,Zhang, Dayi,&Zhang, Gan.(2024).Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC).ENVIRONMENT INTERNATIONAL,185,11. |
| MLA | Li, Jibing,et al."Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC)".ENVIRONMENT INTERNATIONAL 185(2024):11. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment