Knowledge Management System Of Guangzhou Institute of Geochemistry，CAS
| Lian, Luning1,2,3; Xing, Yi1,2,3; Zhang, Dayi4; Jiang, Longfei5; Song, Mengke6; Jiang, Bo1,2,3,7 | |
| Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders | |
| Source Publication | FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
 |
| ISSN | 2095-2201 |
| 2024 | |
| Volume | 18Issue:1Pages:11 |
| DOI | 10.1007/s11783-024-1765-x |
| Language | 英语 |
| WOS Research Area | Engineering ; Environmental Sciences & Ecology |
| Abstract | Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches. |
| Keyword | Stable isotope probing (SIP) Magnetic-nanoparticle mediated isolation (MMI) Dimethoate Biodegradation Cultivation-independent approach |
| WOS ID | WOS:001049186200001 |
| Indexed By | SCI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.gig.ac.cn/handle/344008/74644 |
| Collection | 中国科学院广州地球化学研究所 |
| Corresponding Author | Xing, Yi; Jiang, Bo |
| Affiliation | 1.Univ Sci & Technol Beijing, Sch Energy & Environm Engn, Beijing 100083, Peoples R China 2.Univ Sci & Technol Beijing, Beijing Key Lab Resource oriented Treatment Ind Po, Beijing 100083, Peoples R China 3.Univ Sci & Technol Beijing, Natl Environm & Energy Sci & Technol Int Cooperat, Beijing 100083, Peoples R China 4.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China 6.South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Peoples R China 7.Natl Engn Lab Site Remediat Technol, Beijing 100015, Peoples R China |
| Recommended Citation GB/T 7714 | Lian, Luning,Xing, Yi,Zhang, Dayi,et al. Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING,2024,18(1):11. |
| APA | Lian, Luning,Xing, Yi,Zhang, Dayi,Jiang, Longfei,Song, Mengke,&Jiang, Bo.(2024).Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders.FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING,18(1),11. |
| MLA | Lian, Luning,et al."Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders".FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING 18.1(2024):11. |
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