Knowledge Management System Of Guangzhou Institute of Geochemistry，CAS
An, Jing1; Jiang, Jingjing1; Tang, Waner1; Zhong, Yufang1; Ren, Guofa1; Shang, Yu1; Yu, Zhiqiang2,3,4
| |
| Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells | |
| Source Publication | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| ISSN | 0147-6513 |
| 2023-09-01 | |
| Volume | 262Pages:10 |
| DOI | 10.1016/j.ecoenv.2023.115160 |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology |
| Abstract | Triphenyl phosphate (TPHP) has been widely used as flame retardants and been detected with increasing frequency in environment. TPHP can transform into mono-hydroxylated phosphate (OH-TPHP) and diester diphenyl phosphate (DPHP) through biotransformation. So far, information on the cytotoxicity and molecular regulatory mechanisms of TPHP metabolites are still limit. This study investigated the adverse effects of TPHP, OH-TPHP, and DPHP in HepG2 cells in terms of cell proliferation, lactate dehydrogenase release, reactive oxygen species generation, and mitochondrial membrane potential. The transcriptomic changes were measured using RNA sequencing, and bioinformatics characteristics including biological functions, signal pathways and proteinprotein interaction were analyzed to explore the potential molecular mechanisms. Results displayed that the order of cytotoxicity was OH-TPHP> TPHP> DPHP. The prioritized biological functions changes induced by TPHP and OH-TPHP were correlated with lipid metabolism. Significant lipid accumulation was observed as confirmed by increased total cholesterol and triglycerides contents, and enhanced oil red O staining. Enrichment of PPAR & alpha;/& gamma; and down-stream genes suggested the participation of PPARs signal pathway in lipid metabolism disorder. In addition, TPHP and OH-TPHP induced endoplasmic reticulum stress (ERS), which was further confirmed by the ERS inhibitor experiment. In general, TPHP and OH-TPHP had obvious cytotoxic effects in HepG2 cells. PPARs signal pathway and endoplasmic reticulum stress may be involved in the lipid metabolism disorder induced by TPHP and OH-TPHP. |
| Keyword | Triphenyl phosphate (TPHP) OH-TPHP DPHP Lipid metabolism PPARs signal pathway Endoplasmic reticulum stress |
| WOS ID | WOS:001032373900001 |
| Indexed By | SCI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.gig.ac.cn/handle/344008/74518 |
| Collection | 有机地球化学国家重点实验室 |
| Corresponding Author | Yu, Zhiqiang |
| Affiliation | 1.Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangdong Prov Key Lab Environm Protect & Resource, Guangzhou 510640, Peoples R China 3.CAS Ctr Excellence Deep Earth Sci, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Geochem, CAS Ctr Excellence Deep Earth Sci, Guangzhou 510640, Peoples R China |
| Recommended Citation GB/T 7714 | An, Jing,Jiang, Jingjing,Tang, Waner,et al. Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,262:10. |
| APA | An, Jing.,Jiang, Jingjing.,Tang, Waner.,Zhong, Yufang.,Ren, Guofa.,...&Yu, Zhiqiang.(2023).Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,262,10. |
| MLA | An, Jing,et al."Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 262(2023):10. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment