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抗诺氟沙星多克隆抗体及其制备方法和应用
崔剑岚; 彭先芝; 黄秋鑫; 余以义; 张坤
2010-05-18
Country中国
Patent NumberZL201010181727.6
Rights Holder中国科学院广州地球化学研究所
Contribution Rank1
Abstract本发明公开了一种抗诺氟沙星多克隆抗体及其制备方法和应用。该抗体是通过以下方法制备的:a)免疫原的制备:以诺氟沙星为半抗原,通过N-羟基琥珀酰亚胺活性酯法(NHS)将诺氟沙星与牛血清蛋白(BSA)偶联,得到免疫原;b)多克隆抗体的制备:将免疫原免疫动物,取动物血清,用免疫球蛋白A亲和层析法对血清进行纯化,最后获得的抗体,即为抗诺氟沙星多克隆抗体。该抗诺氟沙星多克隆抗体具有单一性高、灵敏度高、特异性强、并且能长期保存,能高效结合诺氟沙星的优点,因此可应用于检测诺氟沙星在水环境中的残留。
Date Available2010-10-13
Language中文
Application NumberCN201010181727.6
Open (Notice) NumberCN101857637A
Document Type专利
Identifierhttp://ir.gig.ac.cn/handle/344008/35844
Collection专利
Recommended Citation
GB/T 7714
崔剑岚,彭先芝,黄秋鑫,等. 抗诺氟沙星多克隆抗体及其制备方法和应用. ZL201010181727.6[P]. 2010-05-18.
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